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61.
Tsuyoshi Satoh Tadashi Watanabe Masanori Tadokoro Junichi Sakamoto Hiroki Murayama Katsuki Itoh Sadayuki Sakuma Hiroshi Takagi 《Cancer science》1992,83(4):379-386
Anti-carcinoembryonic antigen monoclonal antibody (MAb) CEA102 was produced by immunization with purified CEA and the specific accumulation of radiolabeled CEA102 in colorectal cancers was investigated by autoradiography of surgical specimens using Fuji Computed Radiography (FCR). Five patients with colorectal cancer were injected intravenously with 131 I-labeled intact CEA102 or its F(ab')2 . Primary tumor and liver metastases were successfully detected by external scanning with a gamma camera in 4 cases. Autoradiographic study of the surgical specimens using FCR showed predominant localization of 131 I-labeled CEA102 in primary tumors and liver metastases in all cases. Even a small liver metastasis (0.5 cm) was clearly visualized in the autoradiogram by FCR. The pixel distribution curves of the density of the respective tissues in the autoradiograms by FCR showed the heterogeneity of the distribution of administered radiolabeled MAb in individual tumors, but the density of the tumors was higher than that of the normal tissues. In the quantitative distribution analysis of CEA102, the uptake of the primary tumor (mean 1.10%ID/kg) was ten-fold greater than that of the normal colon mucosa (mean G.10%ID/kg). These results revealed that the application of MAb has great potential in radioimmunodetection as well as in antibody-directed therapy. 相似文献
62.
A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3 -specific peptide (H2 N-12 WRPTSAEGDFELGISSKQKRKKTKTVKMI41 G-COOH) and hybridizing the primed mouse splenic B cells with X63-Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross-react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues. 相似文献
63.
Y. W. Loke A. King T. Burrows L. Gardner M. Bowen S. Hiby S. Howlett N. Holmes D. Jacobs 《Tissue antigens》1997,50(2):135-146
A monoclonal antibody to HLA-G has been generated by immunizing HLA-A2.1/human β2 -microglobulin (β2 m) double transgenic mice with murine L cells transfected with both human β2 m and HLA-G. This monoclonal antibody, designated as G233, has been found not to cross-react with other HLA class I antigens when tested on numerous cell lines by flow cytometry. With immunohistology, all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells) were stained. An extensive range of adult and fetal tissues was also tested but none reacted with monoclonal antibody G233, including those previously reported to express HLA-G mRNA, indicating that the protein has a highly restricted distribution. Failure to detect HLA-G in the fetal thymus raises the question as to how T-cell tolerance to this antigen is induced. Immunoprecipitation of trophoblast surface proteins with monoclonal antibody G233 revealed a heavy chain of 39 kDa and a light chain of 12 kDa, indicating that HLA-G expressed on the surface of trophoblast is complexed with p2m. However, sequential immunoprecipitation with monoclonal antibody W6/32 followed by monoclonal antibody G233 continued to detect a residual band of 39 kDa, suggesting that trophoblast surface HLA-G may also occur as free heavy chains not associated with p2m. Immunoprecipitation followed by two dimensional gel electrophoresis showed that monoclonal antibody G233 recognizes several iso-forms of HLA-G from trophoblast similar to the characteristic spot array previously described for HLA-G. This monoclonal antibody G233 will be highly useful in future experiments to elucidate the function of HLA-G. 相似文献
64.
作者以ABEI-CoCl_2-H_2O_2作为化学发光指示系统,用固相竞争法检测58例白细胞减少症患者、41例无白细胞减少症患者及32例正常人等的粒细胞抗体IgG。判断标准采用正常人血清化学发光强度指数法。实验结果表明,白细胞减少症患者血清中粒细胞抗体阳性率明显高于无白细胞减少症患者(P<0.005)。本法敏感性为43.1%,特异性为90.2%,准确性为62.6%,是一种灵敏而准确的方法,能将粒细胞包被试管在室温下较长时间保存,以供临床监测粒细胞抗体,大大缩短检测时间。 相似文献
65.
本文动用自制t-PA单克隆抗体建立t-PA:Aa夹心ELISA法。结合t-PA:A、PAI:A测定,对50例正常人,87例肝病,47例冠心病和19例深静脉血栓 成患者的血浆t-PA:Ag、t-PA:A、PAI:A水平进行了研究。 相似文献
66.
Induction of type II collagen-specific antibody production in blood lymphocyte cultures of rhesus monkeys (Macaca mulatta) with collagen-induced arthritis using the immobilized native antigen. 下载免费PDF全文
Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a heating step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced. 相似文献
67.
K. CHEUNG R. URECH L. TAYLOR P. DUFFY D. RADFORD 《Journal of paediatrics and child health》1991,27(5):312-313
The potential application of the Digoxin Fab antibody (Wellcome Digibind) in the clinical management of plant poisoning was investigated. The cardiac glycoside contents of various Australian plants were studied using immunoassay techniques. The cross-reactions of the Fab antibody and two digoxin assay antibodies against extracts of these plants were also studied. Results obtained indicated that the Digibind antibody cross-reacted with a wide range of glycosides contained in Australian plants and therefore could be of use in the treatment of life-threatening plant poisoning. 相似文献
68.
E. Munteis J. F. Cano J. A. Flores J. E. Martinez-Rodriguez M. Miret J. Roquer 《European journal of neurology》2007,14(9):1048-1052
The aim of the study was to determine the prevalence of thyroid autoimmune disorders in a cohort of untreated multiple sclerosis (MS) patients and compare it with a stratified sample of an adult population. We prospectively studied 93 untreated MS patients. The control group included 401 healthy subjects selected by stratified sampling in a non-iodine-deficient area. Antithyroid antibodies (ATA) (antibodies against peroxidase and thyroglobulin) were considered positive at titres ≥149 IU/ml. Antibodies were positive in 11 MS patients (11.8%; 95% CI 5.3–18.4%). This prevalence was five times higher ( P = 0.0001) when compared with that in the control population. We found six cases with subclinical hypothyroidism (6.45%; 95% CI 11.4–1.5) in contrast to 2.24% in the control group. Comparing MS with positive and negative ATA, there was a non-significant, slightly higher frequency of low Expanded Disability Status Scale (EDSS) score in the ATA-positive group (81% vs. 73.2%). One year after start of interferon (IFN) treatment, only one patient developed subclinical thyroid dysfunction. MS patients have a higher prevalence of ATA compared with the general population. An initial ATA and thyroid-stimulating hormone (TSH) determination is recommended in all MS patients. A periodic assessment of thyroid function during IFN treatment only seems to be justified in those cases where positive ATA or dysfunction is present before treatment. 相似文献
69.
同型半胱氨酸及抗磷脂抗体与深静脉血栓形成和复发关系的研究 总被引:2,自引:0,他引:2
目的通过检测深静脉血栓形成(DVT)患者血浆中的同型半胱氨酸(Hey)和抗磷脂抗体(APLA)水平,探讨DVT形成和复发的因素,并寻找DVT复发的预报因子。方法选取2001年1月至2003年4月期间哈尔滨医科大学附属第二医院血管外科收治的60例DVT患者,其中初发组和复发组各30例,另随机选取门诊就诊经证实无DVT的下肢静脉曲张或深静脉功能不全的30例患者作为对照组,同时选取复发DVT的一级亲属组30例。用荧光偏振免疫法测定Hey,用酶联免疫吸附试验(ELISA)检测APLA中的抗心磷脂抗体(AcLA)水平(IgG、IgM型)并测定狼疮抗体(LA)阳性率。结果初发与复发DVT组患者血浆中的Hey和ACLA-IgG及ACLA-IgM水平均高于对照组和复发DVT一级亲属组,差异有统计学意义(P〈0.01)。复发DVT的一级亲属组的血浆中的Hey水平高于对照组(P〈0.05)。各组的LA阳性率差异无统计学意义(P〉0.05)。结论DVT患者血浆中Hey和APLA均升高,Hey和APLA与深静脉血栓形成有密切关系。Hey升高与DVT复发有关,可能是DVT复发的预报因子。 相似文献
70.